Scientific labs can perform this service by request for customers and researchers can also do it in their own facilities, if they have the necessary equipment. Gene cloning is the replication of certain type of genes from a certain part of the cell/ DNA to propagate a certain desirable genetic trait. Isolation of DNA (gene of interest) fragments to be cloned 2. Cloned cells may one day be used to treat a damaged heart or neurons. In which fragment of DNA is cloned. While the purpose of cloning is to create an exact replica – if scientists cloned a human that appears identical to the original – it raises the questions as to whether the cloned human is an individual separate from the original and is due the same rights as any other human. The following points highlight the seven main steps involved in gene cloning. Some of the steps are: 1. Insertion of Isolated DNA into the a suitable vector to form the recombinant DNA 3. Introduction of the recombinant DNA into a suitable organism known as host and other steps too. DNA cloning can be described as the process of making multiple, identical copies of a particular piece of the genetic material or DNA fragment. Gene cloning is also known as DNA cloning. The genetic material will be identical to their own. In molecular biology, ligation is the joining of two nucleic acid fragments through the action of an enzyme. By using cloning a person is able to find out if he or she has inherited a gene on a chromosome from an affected parent by a procedure called genetic screening. Gene cloning is the replication of DNA fragments by the use of a self-replicating genetic material. Testing new medicines. By cloning a person’s own body cells, scientists hope that a cloned stem cell line will not be rejected by the patient receiving the cells. Gene cloning allows researchers to generate copies of a gene of interest for further study, use in medical testing, or therapy. The copied material is called a clone. This process allows individuals to find out if they have an affected gene or not, if the individual does have an affected gene then they can use strategies to help prevent the disease or make the symptoms/disease itself weaker. In a typical DNA cloning process, the gene or other the target DNA fragment is first inserted into a circular piece of DNA known as a plasmid. Isolation and obtaining of desired gene The gene to be isolated is identified in the DNA. It involves a series of stages to separate out the gene and propagate it. Cloning is the process of taking genetic information from one living thing and creating identical copies of it.
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